The posttraumatic response of CD4+ regulatory T cells is modulated by direct cell-cell contact via CD40L- and P-selectin-dependent pathways

Rupp M, Benjamin Bergmann C, Jung S, et al. The posttraumatic response of CD4+ regulatory T cells is modulated by direct cell-cell contact via CD40L- and P-selectin-dependent pathways. Central European Journal of Immunology. 2021;46(3):283-294. doi:10.5114/ceji.2021.109171.

APA

Rupp, M., Benjamin Bergmann, C., Jung, S., Bock, M., Biberthaler, P.,  & Heimann, L. et al. (2021). The posttraumatic response of CD4+ regulatory T cells is modulated by direct cell-cell contact via CD40L- and P-selectin-dependent pathways. Central European Journal of Immunology, 46(3), 283-294. https://doi.org/10.5114/ceji.2021.109171

Chicago

Rupp, Marco-Christopher, Christian Benjamin Bergmann, Sonja Jung, Matthias Bock, Peter Biberthaler, Laura Heimann,  and Marc Hanschen. 2021. "The posttraumatic response of CD4+ regulatory T cells is modulated by direct cell-cell contact via CD40L- and P-selectin-dependent pathways". Central European Journal of Immunology 46 (3): 283-294. doi:10.5114/ceji.2021.109171.

Harvard

Rupp, M., Benjamin Bergmann, C., Jung, S., Bock, M., Biberthaler, P., Heimann, L.,  and Hanschen, M. (2021). The posttraumatic response of CD4+ regulatory T cells is modulated by direct cell-cell contact via CD40L- and P-selectin-dependent pathways. Central European Journal of Immunology, 46(3), pp.283-294. https://doi.org/10.5114/ceji.2021.109171

MLA

Rupp, Marco-Christopher et al. "The posttraumatic response of CD4+ regulatory T cells is modulated by direct cell-cell contact via CD40L- and P-selectin-dependent pathways." Central European Journal of Immunology, vol. 46, no. 3, 2021, pp. 283-294. doi:10.5114/ceji.2021.109171.

Vancouver

Rupp M, Benjamin Bergmann C, Jung S, Bock M, Biberthaler P, Heimann L et al. The posttraumatic response of CD4+ regulatory T cells is modulated by direct cell-cell contact via CD40L- and P-selectin-dependent pathways. Central European Journal of Immunology. 2021;46(3):283-294. doi:10.5114/ceji.2021.109171.

PlumX metrics:

CD4+ FoxP3+ regulatory T cells (CD4+ Tregs) are important for the posttraumatic anti-inflammatory host response. As described previously, platelets are able to modulate CD4+ Treg activity in a reciprocally activating interaction following injury. The underlying mechanisms of the posttraumatic interaction between platelets and CD4+ Tregs remain unclear. We investigated the potential influence of CD40L and P-selectin, molecules known to be involved in direct cell contact of these cell types. In a murine burn injury model, the potential interaction pathways were addressed using CD40L- and P-selectin-deficient mice. Draining lymph nodes were harvested following trauma (1 h) and following a sham procedure. Early rapid activation of CD4+ Tregs was assessed by phospho-flow cytometry (signaling molecules (p)PKC- and (p)ZAP-70). Platelet function was analyzed performing rotational thromboelastometry (ROTEM). We hypothesized that disruption of the direct cell-cell contact via CD40L and P-selectin would affect posttraumatic activation of CD4+ Tregs and influence the hemostatic function of platelets. Indeed, while injury induced early activation of CD4+ Tregs in wild-type mice (ZAP-70: p = 0.13, pZAP-70: p < 0.05, PKC-: p < 0.05, pPKC-: p < 0.05), disruption of CD40L-dependent interaction (ZAP-70: p = 0.57, pZAP-70: p = 0.68, PKC-: p = 0.68, pPKC-: p = 0.9) or P-selectin-dependent interaction (ZAP-70: p = 0.78, pZAP-70: p = 0.58, PKC-: p = 0.81, pPKC-: p = 0.73) resulted in reduced posttraumatic activation. Furthermore, hemostatic function was impaired towards hypocoagulability in either deficiency. Our results suggest that the posttraumatic activation of CD4+ Tregs and hemostatic function of platelets are affected by direct cell-cell-signaling via CD40L and P-selectin.

keywords:

The posttraumatic response of CD4+ regulatory T cells is modulated by direct cell-cell contact via CD40L- and P-selectin-dependent pathways

Marco-Christopher Rupp 1 , Christian Benjamin Bergmann 1 , Sonja Jung 1 , Matthias Bock 1 , Peter Biberthaler 1 , Laura Heimann 1 , Marc Hanschen 1

CD4+ regulatory T cells, platelets, trauma, adaptive immune response, hemostasis, cell communication

Marco-Christopher Rupp

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Christian Benjamin Bergmann

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Sonja Jung

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Matthias Bock

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Peter Biberthaler

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Laura Heimann

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Marc Hanschen

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