The impact of physical training on neutrophil extracellular traps
in young male athletes – a pilot study

Joanna Orysiak; Jitendra K. Tripathi; Klaudia K. Brodaczewska; Atul Sharma; Konrad Witek; Dariusz Sitkowski; Jadwiga Malczewska-Lenczowska

doi:10.5114/biolsport.2021.101117

eISSN: 2083-1862
ISSN: 0860-021X

Biology of Sport

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3/2021
vol. 38

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abstract:

Original paper

The impact of physical training on neutrophil extracellular traps in young male athletes – a pilot study

Joanna Orysiak

^{1, 2}

,

Jitendra K. Tripathi

³

,

Klaudia K. Brodaczewska

⁴

,

Atul Sharma

³

,

Konrad Witek

⁵

,

Dariusz Sitkowski

⁶

,

Jadwiga Malczewska-Lenczowska

¹

Department of Nutrition Physiology and Dietetics, Institute of Sport-National Research Institute, Warsaw, Poland
Department of Ergonomics, Central Institute for Labour Protection-National Research Institute, Warsaw, Poland
Department of Biomedical Sciences, University of North Dakota School of Medicine and Health Sciences, Grand Forks, USA
Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine, Warsaw, Poland
Department of Biochemistry, Institute of Sport-National Research Institute, Warsaw, Poland
Department of Physiology, Institute of Sport-National Research Institute, Warsaw, Poland

Biol Sport. 2021;38(3):459–464.

DOI: https://doi.org/10.5114/biolsport.2021.101117

Online publish date: 2020/11/30

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Neutrophils are an important component of the innate immune response against various pathogens. However, there is a lack of research concerning the effects of short intensive training on neutrophil functions, especially neutrophil extracellular traps (NET) formation. The study aim was to determine the effects of a 19-day training cycle on innate immunity among young male athletes. Six male ice hockey players (< 20 years old) from the Polish national team were monitored across a five-day training camp and after a return to normal club training. The first blood collection took place before training (T1), the second after the training camp (T2) and the third 14 days later (T3). The counts/concentrations of blood biochemical, immune and endocrine markers were compared across each training period. Creatine kinase activity tended to increase at T2 (546 ± 216 U·L-1) when compared to T1 (191 ± 111 U·L-1; p=0.063). Neutrophil extracellular traps formation and neutrophil counts also differed between training periods (p=0.042 and p=0.042, respectively). Neutrophil counts tended to decrease, in contrast to NET formation which tended to rise, at T2 in comparison to T1 (2.51 ± 0.45 vs 3.04 ± 0.47 109 ·L-1; 24 ± 13 vs 8 ± 15%, respectively). No significant differences in other leucocyte counts were observed. A short period of intensive training was accompanied by some muscle damage and inflammation, as evidenced by CK and NET up-regulation, whilst neutrophil counts were diminished in the blood. Thus, neutrophils and NET could be involved in muscle damage and local inflammatory processes following intensive physical training in young male athletes

keywords:

The impact of physical training on neutrophil extracellular traps in young male athletes – a pilot study

Joanna Orysiak 1, 2 , Jitendra K. Tripathi 3 , Klaudia K. Brodaczewska 4 , Atul Sharma 3 , Konrad Witek 5 , Dariusz Sitkowski 6 , Jadwiga Malczewska-Lenczowska 1

Innate immunity, Intensive training, Neutrophils, Muscle damage, Inflammatory markers

Joanna Orysiak

^{1, 2}

,

Jitendra K. Tripathi

³

,

Klaudia K. Brodaczewska

⁴

,

Atul Sharma

³

,

Konrad Witek

⁵

,

Dariusz Sitkowski

⁶

,

Jadwiga Malczewska-Lenczowska

¹