miR-22 negatively regulating NOD-like receptor protein 3 gene in the proliferation, invasion, and migration of malignant melanoma cells

Hongyan Liu; Wenlian Huang; Xiaoshu Pu; Yi Chen; Yinbin Zheng; Ying Lei; Ting Jiang

doi:10.5114/ada.2024.140521

eISSN: 2299-0046
ISSN: 1642-395X

Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii

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3/2024
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abstract:

Original paper

miR-22 negatively regulating NOD-like receptor protein 3 gene in the proliferation, invasion, and migration of malignant melanoma cells

Hongyan Liu

¹

,

Wenlian Huang

²

,

Xiaoshu Pu

¹

,

Yi Chen

³

,

Yinbin Zheng

⁴

,

Ying Lei

¹

,

Ting Jiang

¹

Department of Burn Plastic and Aesthetic Surgery, Nanchong Central Hospital, Second Clinical Medical College of North Sichuan Medical College, Nanchong, Sichuan Province, China
Department of Critical Care Medicine, Nanchong Central Hospital, Second Clinical Medical College of North Sichuan Medical College, Nanchong, Sichuan Province, China
Department of Stomatology, Nanchong Central Hospital, Second Clinical Medical College of North Sichuan Medical College, Nanchong, Sichuan Province, China
Department of Thoracic Surgery, Nanchong Central Hospital, Second Clinical Medical College of North Sichuan Medical College, Nanchong, Sichuan Province, China

Adv Dermatol Allergol 2024; XLI (3): 284-291

DOI: https://doi.org/10.5114/ada.2024.140521

Online publish date: 2024/04/25

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AMA

Liu H, Huang W, Pu X, et al. miR-22 negatively regulating NOD-like receptor protein 3 gene in the proliferation, invasion, and migration of malignant melanoma cells. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2024;41(3):284-291. doi:10.5114/ada.2024.140521.

APA

Liu, H., Huang, W., Pu, X., Chen, Y., Zheng, Y.,  & Lei, Y. et al. (2024). miR-22 negatively regulating NOD-like receptor protein 3 gene in the proliferation, invasion, and migration of malignant melanoma cells. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 41(3), 284-291. https://doi.org/10.5114/ada.2024.140521

Chicago

Liu, Hongyan, Wenlian Huang, Xiaoshu Pu, Yi Chen, Yinbin Zheng, Ying Lei,  and Ting Jiang. 2024. "miR-22 negatively regulating NOD-like receptor protein 3 gene in the proliferation, invasion, and migration of malignant melanoma cells". Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii 41 (3): 284-291. doi:10.5114/ada.2024.140521.

Harvard

Liu, H., Huang, W., Pu, X., Chen, Y., Zheng, Y., Lei, Y.,  and Jiang, T. (2024). miR-22 negatively regulating NOD-like receptor protein 3 gene in the proliferation, invasion, and migration of malignant melanoma cells. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 41(3), pp.284-291. https://doi.org/10.5114/ada.2024.140521

MLA

Liu, Hongyan et al. "miR-22 negatively regulating NOD-like receptor protein 3 gene in the proliferation, invasion, and migration of malignant melanoma cells." Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, vol. 41, no. 3, 2024, pp. 284-291. doi:10.5114/ada.2024.140521.

Vancouver

Liu H, Huang W, Pu X, Chen Y, Zheng Y, Lei Y et al. miR-22 negatively regulating NOD-like receptor protein 3 gene in the proliferation, invasion, and migration of malignant melanoma cells. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2024;41(3):284-291. doi:10.5114/ada.2024.140521.

Introduction:
Malignant melanoma (MM) is a highly aggressive skin tumour.

Aim:
To investigate whether miR-22 is involved in the proliferation, invasion, and migration of melanoma cells (MCs) by negatively regulating NOD-like receptor protein 3 (NLRP3) gene.

Material and methods:
Human MCs (WM239a) and human epidermal melanocytes (HEM) were used as study material. The expression levels of miR-22 and NLRP3 were detected by qRT-PCR. The expression of NLRP3 protein was determined by Western blot (WB) analysis. The effects of miR-22 and NLRP3 on the proliferation, invasion, and migration of MCs were evaluated by cell counting kit-8 (CCK-8), Transwell cell invasion assay, and scratch assay.

Results:
The expression of miR-22 was clearly lower in WM239a than in HEM. Up-regulation of miR-22 expression in WM239a clearly raised the expression of miR-22, Caspase-1, and E-cadherin and the apoptotic rate of WM239a; however, the levels of interleukin-1b (IL-1b) and NLRP3, cell proliferation activity, invasion and migration ability were clearly decreased. The negative regulation of NLRP3 by miR-22 may play a major role in activities of MM.

Conclusions:
Further studies will help to reveal the molecular details of this regulatory mechanism and provide new therapeutic strategies.

keywords:

miR-22 negatively regulating NOD-like receptor protein 3 gene in the proliferation, invasion, and migration of malignant melanoma cells

Hongyan Liu 1 , Wenlian Huang 2 , Xiaoshu Pu 1 , Yi Chen 3 , Yinbin Zheng 4 , Ying Lei 1 , Ting Jiang 1

miR-22, NLRP3 gene, MM cells, CCK-8, Transwell cell invasion

Hongyan Liu

¹

,

Wenlian Huang

²

,

Xiaoshu Pu

¹

,

Yi Chen

³

,

Yinbin Zheng

⁴

,

Ying Lei

¹

,

Ting Jiang

¹