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eISSN: 2719-3209
ISSN: 0023-2157
Klinika Oczna / Acta Ophthalmologica Polonica
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1/2009
vol. 111
 
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abstract:
Original paper

In vitro photodynamic properties of methylene blue in a combination with laser illumination at 630 nm concerning Candida albicans

Nataliya Pasyechnikova
1
,
Olexandra Zborovskaya
1
,
Taras Kustrin
1

  1. Laboratory of Microbiology and Department of Uveitis, The Filatov`s Institute of Eye Diseases and Tissue Therapy of AMS of Ukraine
Klinika Oczna 2009, 111 (1): 15-17
Online publish date: 2009/04/05
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The study had to define influence of combined applications of laser radiation and methylene blue (MB) on pathogenic culture of Candida albicans in vitro. The experimental study was done at standard techniques of method of cultivations in a broth. The laser irradiation of cultures was done at once after addition of MB in concentration 0.05%, 0.1% and 0.2%. During studying action of MB in dark, influence of MB to the growth of test-shtam without laser radiation, were prepared fluid Gissa’s broth with glucose without Andrede’s indicator. Activation of MB was done by laser with wave length 630 nm during 3 or 5 min. All experiments were passed in 4 parallels and 3 repeats. Maximal suppression of growth of microorganisms was noted in group with using 0.1% MB with laser radiation 3 minutes without centrifugation after 24 hours. Maximal suppression of growth was noted in group after centrifugation with 0.05% MB with exposure of laser 3 min. after 48 hours. Sensitivity of pathogenic culture of Candida albicans to application of МB and laser raises accordingly to increase of concentration of MB.
keywords:

methylene blue, photosensitizer, Candida albicans

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