Shift in skin microbiota of children with atopic dermatitis
after topical gentian violet application

Joanna Krzysiek; Joanna Żurawska-Olszewska; Izabela Szczerba; Aleksandra Lesiak; Dorota Pastuszak-Lewandoska; Janina Grzegorczyk; Magdalena Ciążyńska; Joanna Narbutt

doi:10.5114/ada.2023.126534

eISSN: 2299-0046
ISSN: 1642-395X

Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii

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2/2023
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abstract:

Original paper

Shift in skin microbiota of children with atopic dermatitis after topical gentian violet application

Joanna Krzysiek

¹

,

Joanna Żurawska-Olszewska

²

,

Izabela Szczerba

²

,

Aleksandra Lesiak

¹

,

Dorota Pastuszak-Lewandoska

²

,

Janina Grzegorczyk

²

,

Magdalena Ciążyńska

^{1, 2}

,

Joanna Narbutt

¹

Department of Dermatology, Pediatric and Oncology Dermatology, Medical University of Lodz, Lodz, Poland
Microbiology and Laboratory Medical Immunology, Medical University of Lodz, Lodz, Poland

Adv Dermatol Allergol 2023; XL (2): 308-314

DOI: https://doi.org/10.5114/ada.2023.126534

Online publish date: 2023/04/13

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AMA

Krzysiek J, Żurawska-Olszewska J, Szczerba I, et al. Shift in skin microbiota of children with atopic dermatitis
after topical gentian violet application. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2023;40(2):308-314. doi:10.5114/ada.2023.126534.

APA

Krzysiek, J., Żurawska-Olszewska, J., Szczerba, I., Lesiak, A., Pastuszak-Lewandoska, D.,  & Grzegorczyk, J. et al. (2023). Shift in skin microbiota of children with atopic dermatitis
after topical gentian violet application. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 40(2), 308-314. https://doi.org/10.5114/ada.2023.126534

Chicago

Krzysiek, Joanna, Joanna Żurawska-Olszewska, Izabela Szczerba, Aleksandra Lesiak, Dorota Pastuszak-Lewandoska, Janina Grzegorczyk,  and Magdalena Ciążyńska et al. 2023. "Shift in skin microbiota of children with atopic dermatitis
after topical gentian violet application". Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii 40 (2): 308-314. doi:10.5114/ada.2023.126534.

Harvard

Krzysiek, J., Żurawska-Olszewska, J., Szczerba, I., Lesiak, A., Pastuszak-Lewandoska, D., Grzegorczyk, J., Ciążyńska, M.,  and Narbutt, J. (2023). Shift in skin microbiota of children with atopic dermatitis
after topical gentian violet application. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 40(2), pp.308-314. https://doi.org/10.5114/ada.2023.126534

MLA

Krzysiek, Joanna et al. "Shift in skin microbiota of children with atopic dermatitis
after topical gentian violet application." Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, vol. 40, no. 2, 2023, pp. 308-314. doi:10.5114/ada.2023.126534.

Vancouver

Krzysiek J, Żurawska-Olszewska J, Szczerba I, Lesiak A, Pastuszak-Lewandoska D, Grzegorczyk J et al. Shift in skin microbiota of children with atopic dermatitis
after topical gentian violet application. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2023;40(2):308-314. doi:10.5114/ada.2023.126534.

Introduction:
Increasing usage of antimicrobial agents may contribute to bacterial resistance in atopic dermatitis (AD). In this case an alternative topical treatment might be gentian violet (GV), suggested for its antibacterial and antifungal properties.

Aim:
To assess the microbial composition of lesional skin in children with AD and a control group aged 2–12 years, before and after 3 days of 2% aqueous GV application.

Material and methods:
Skin samples were taken from 30 AD patients and 30 healthy controls aged 2–12 years. The procedure was done two times – before and after 3 days of 2% aqueous GV application. The material was collected from skin lesions in the cubital fossa using 25 cm² impression plates, containing CHROMagar Staph aureus and CHROMagar Malassezia. After the incubation period, the grown colonies were counted and identified by the Phoenix BD testing system.

Results:
The results revealed a statistically significant reduction in total counts of bacteria in both groups of children after GV application (p < 0.05). The significant decrease in the number was seen in Staphylococcus spp. (S. aureus, S. capitis, S. haemolyticus, S. cohnii) in AD patients. The number of Staphylococcus spp. was comparable in patients with AD after GV treatment and healthy patients before GV exposure (p = 1.000).

Conclusions:
Our study results show that GV does not damage the skin surface ecosystem and allows the reduction of excessive bacterial counts on eczematous lesions to a ‘safe’ level, similar to that of healthy children.

keywords:

Shift in skin microbiota of children with atopic dermatitis after topical gentian violet application

Joanna Krzysiek 1 , Joanna Żurawska-Olszewska 2 , Izabela Szczerba 2 , Aleksandra Lesiak 1 , Dorota Pastuszak-Lewandoska 2 , Janina Grzegorczyk 2 , Magdalena Ciążyńska 1, 2 , Joanna Narbutt 1

atopic dermatitis, microbiome, gentian violet

Joanna Krzysiek

¹

,

Joanna Żurawska-Olszewska

²

,

Izabela Szczerba

²

,

Aleksandra Lesiak

¹

,

Dorota Pastuszak-Lewandoska

²

,

Janina Grzegorczyk

²

,

Magdalena Ciążyńska

^{1, 2}

,

Joanna Narbutt

¹